ABSTRACT
Backgroud: Liver is an important metabolic organ. It has wide range of functions including detoxification, storage of glycogen, vitamins A, D and B12, production of several coagulation factors, growth factors such as insulin-like growth factor-1 (IGF-1), angiotensinogen, and biochemicals necessary for digestion (bile). Its damage occurs due to its multidimensional functions, various xenobiotics and oxidative stress leading to distortion of all of its functions. Oyster mushroom which is excellently edible and nutritious has got free radical scavenging activity, and so may be considered as a hepatoprotective agent. Objective: To observe the hepatoprotective effect of Oyster mushroom (Pleurotus florida) against paracetamol induced liver damage in Wistar albino rats. Materials and Methods: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st July 2009 to 30th June 2010. Thirty four Wistar albino rats, aged 90 to 120 days, weighing between 150 to 210 grams were used for the study. After acclimatization for 14 days, they were divided into two groups –– control group (Group A) and experimental group (Group B, mushroom-pretreated and paracetamol-treated group). Control group was again subdivided into Group A1 (baseline control group) and Group A2 (paracetamol-treated control group). Animals of all groups received basal diet for 30 consecutive days. In addition, Group A1 rats received propylene glycol (2 mL/kg body weight orally) only on 30th day, Group A2 rats received single dose of paracetamol suspension (750 mg/kg body weight orally) only on 30th day and Group B rats received mushroom extract (200 mg/kg body weight orally) for 30 consecutive days and paracetamol suspension (750 mg/kg body weight orally) only on 30th day. All the animals were sacrificed on 31st day. Then liver specimens were collected. Histology of liver was done by using standard laboratory procedure. Statistical analysis was done by one way ANOVA test by using SPSS version 15.0. Result: In this study, histological examination of liver reveals abnormal histological findings in 100% of rats in paracetamol-treated group (Group A2), almost normal structure in 80% of rats and mild histological changes in 20% rats in mushroom-pretreated and paracetamol-treated group (Group B). Conclusion: The present study reveals the hepatoprotective effect of Oyster mushroom (Pleurotus florida) against paracetamol induced liver damage in Wistar albino rats.
ABSTRACT
Backgroud: Liver is continuously exposed to a variety of toxic agents like drugs and chemicals that may interfere with hepatic function and may cause hepatic damage. Oyster mushroom is excellently edible, nutritious and has got free radical scavenging activity, thereby may be considered as hepatoprotective agent. Objective: To observe the effect of Oyster mushroom on paracetamol induced changes in serum bilirubin and liver tissue protein in rats. Method: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st July 2009 to 30th June 2010. A total number of 34 Wistar albino rats, age ranged from 90 to 120 days, weighing between 150 to 210 grams were selected for the study. After acclimatization for 14 days, they were divided into two groups, control group (Group A) and experimental group (Group B- mushroom pretreated and paracetamol treated group). Control group was again subdivided into group A1 (baseline control) and group A2 (paracetamol treated control group). All groups of animals received basal diet for 30 consecutive days. Group A1 consisted of 10 rats, received propylene glycol (2 ml/kg bw, orally) only on 30th day. Group A2 consisted of 14 rats, received single dose of paracetamol suspension (750 mg/ kg bw, orally) only on 30th day. Group B consisted of 10 rats, received mushroom extract (200 mg/ kg bw, orally) for 30 consecutive days and paracetamol suspension (750 mg/ kg bw, orally) only on 30th day. All the animals were sacrificed on 31st day. Then blood and liver sample were collected. Estimation of serum total bilirubin level and assessment of protein concentration in liver tissue homogenate were done by using standard laboratory kits. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable. Result: The mean serum total bilirubin was significantly (p< 0.001) higher in paracetamol treated group in comparison to that of baseline control group. Again, the mean serum total bilirubin was significantly (p<0.001) lower in mushroom pretreated and paracetamol treated group (experimental group) when compared to that of paracetamol treated group (control). The protein concentration in liver tissue homogenate was significantly (p<0.01) lower in paracetamol treated group in comparison to that of baseline control group. Again, in the liver tissue homogenate protein concentration was significantly (p<0.001) higher in mushroom pretreated and paracetamol treated group (experimental group) when compared to that of paracetamol treated group (control). Conclusion: The present study revealed that Oyster mushroom can protect liver tissue against paracetamol induced liver damage.
ABSTRACT
Background: Liver damage can be occurred due to prolonged use of higher doses of some drugs, exposure to some chemicals or infectious agents. But liver protective drugs are not available in modern medicine. Some hepatoprotective herbal medicines are often used in the treatment of liver damage. Objective: This experimental study was carried out to observe the hepatoprotective effect of Oyster mushroom (Pleurotus florida) against paracetamol induced liver damage in Wistar albino rats. Method: This experimental study was carried out in the Department of Physiology, Sir Salimullah Medical College (SSMC), Dhaka from 1st July 2009 to 30th June 2010. A total number of 34 Wistar albino rats, age ranged from 90 to 120 days, weighing between 150 to 210 grams were selected for the study. After acclimatization for 14 days, they were divided into two groups, control group (Group A) and experimental group (Group B- mushroom pretreated and paracetamol treated group). Control group again subdivided into group A1 (baseline control) and group A2 (paracetamol treated control group). All groups of animals received basal diet for 30 consecutive days. Group A1 consisted of 10 rats, received propylene glycol (2 ml/kg bw, orally) only on 30th day. Group A2 consisted of 14 rats, received single dose of paracetamol suspension (750 mg/ kg bw, orally) only on 30th day. Group B consisted of 10 rats, received mushroom extract (200 mg/ kg bw, orally) for 30 consecutive days and paracetamol suspension (750 mg/ kg bw, orally) only on 30th day. All the animals were sacrificed on 31st day. Then blood and liver samples were collected. Initial body weight, final body weight and liver weight were measured. Then measurement of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum and assessment of malondialdehyde (MDA) concentration in liver tissue homogenate were done by using standard laboratory kits. The statistical analysis was done by one way ANOVA and Bonferroni test as applicable. Result: The mean serum AST, ALT levels and in the liver tissue MDA concentration were significantly (p<0.001) higher in paracetamol treated group in comparison to those of baseline control group. Again, the mean serum AST (p<0.05), ALT (p<0.05) levels and in the liver tissue homogenate MDA concentration (p<0.001) were significantly lower in mushroom pretreated and paracetamol treated group (experimental group) when compared to those of only paracetamol treated group (control). Conclusion: This study reveals that Oyster mushroom (Pleurotus florida) which is excellently edible and nutritious, may have some hepatoprotective role.